Cancer Immunology Research Strategies for Multiplexed Biomarker Discovery ~ Translational studies in Quantitative Digital Pathology utilizing novel staining and imaging techniques
The MD Anderson Flow Cytometry & Cellular Imaging Core Facility and PerkinElmer are pleased to invite you to a lively discussion regarding advanced quantitative digital pathology techniques for Cancer research. This comprehensive seminar will highlight techniques and technologies available through the core facilities in the Department of Leukemia. Please join us for an engaging discussion regarding our joint efforts towards clinical translation. Scientific, application focused presentations will include the following topics:
· Automated Digital Pathology – Systems are available to deliver high quality data where morphological context is preserved down to the sub-cellular level removing autofluorescence & increasing signal-to-noise. Multimodal scanning systems are also offered enabling whole slide imaging in both brightfield and fluorescence along with systems for fabrication of TMA.
· Strategies for Achieving Multiplexed Staining – Achieve robust, specific multiplex tissue biomarker measurement selecting antibodies based on performance rather than species. The typical multiplexed Opal staining procedure may be completed within one day. The method is compatible with the standard IHC workflow in your lab and is amenable to automation. Opal is an iterative process that uses covalent labeling with tyramide signal amplification (TSA™) followed by removal of antibodies. Removal of antibodies has minimal impact on the TSA signal and clears the tissue to be probed with another primary antibody without fear of cross reactivity.
· Quantitative Multiparametric Evaluation of Pathology Images – Bioinformatics approaches are critical to the deconvolution of multiplexed tissue imaging. Software is available for the segmentation and quantitation of single cell data from complex whole tissue. Tools for the interpretation and visualization of this rich multiparametric data will be discussed.
Date: 6/12/14, 9:30am to 12:30pm
Time: 6/12/14, 9:30am to 12:30pm
Location: AT&T Auditorium, Main Building, Floor 2 (B2.4750)
Format: All Employee Meeting
Contact: Joan Zuniga - firstname.lastname@example.org
June 12th FLIER.pdf (PDF, 405 KB)
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