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Detection of somatic variants at 0.1% frequency in cell-free DNA using Oncomine™ cfDNA assays and Ion Torrent sequencing

Detection of somatic variants at 0.1% frequency in cell-free DNA using Oncomine™ cfDNA assays and Ion Torrent sequencing

We demonstrate a research use only workflow that aims to detect variants at low allelic frequencies in cell free

DNA (cfDNA) extracted from liquid biopsy samples. The described workflow includes blood collection, cfDNA

isolation, library preparation, sequencing, and data analysis to enable detection of rare DNA variants in blood

plasma samples using the Oncomine

cfDNA assays.Lung cfDNA Assay. We verified the workflow on

We achieved ~85% detection sensitivity at 0.1% frequency using engineered control samples with >99%

specificity at these low limits of detection using the Oncomine

a set of research samples from matched tumor FFPE and blood plasma collected from research subjects with

NSCLC. Results indicate high sensitivity of the workflow and high concordance between variants detected in

the two types of research samples. This workflow provides a non-invasive approach to monitor cancer status

and evaluate cancer evolution in the future.

Date: 4/28/17, 9:30am to 10:30am
Time: 4/28/17, 9:30am to 10:30am
Location: South Campus Research Building 4
Directions: South Campus Building 4 1901 East Rd Houston, TX 77054 Room: 4SCRB1.1110 (Auditorium)
Format: All Employee Meeting
Speaker: Michael Dell
Sponsor: Chang Gong Liu
Contact: Leticia Evans - (713) 792-0385 - levans3@mdanderson.org