MD Anderson Events
SPECIAL SYMPOSIUM CBSF Club Thursday September 19, 2013 at 3:30 pm
Title: Affinity and Beyond : From screened fragments to optimized leads with Label Free
Speakers: Ronan O’Brien, Head of MicroCal R&D, GE Healthcare, Northampton, MA
Paul Belcher, Development Leader, GE Healthcare, Piscataway, NJ;
Abstract: Biophysical methods are essential tools during the entire drug discovery process. They can be used for optimization of protein constructs and verification of proper and consistent protein folding, identification of optimal assay buffer, screening of fragments and the confirmation of binding to the right or allosteric binding site(s) of the target molecule. You can also measure binding affinity, thermodynamics and kinetics to be able to perform enthalpy and residence time based optimization of leads into candidate drugs. In this work we show how Differential Scanning Calorimetry (DSC) can be used for identification of proper assay buffer and verifying binders in a fragment based lead optimization campaign. Isothermal Titration Calorimetry (ITC) can be used for profiling of fragment hits and for generation of data to guide lead optimization through measuring of binding affinities, additionally the low c approach can identify binders whilst conserving precious target protein. We also demonstrate how an entire fragment screening and verification campaign can be performed with Surface Plasmon Resonance (SPR) biosensors using minute amounts of protein target to identify hits that were than validate via a panel of orthogonal techniques.
Date: 9/19/13, 3:30pm to 5:30pm
Time: 9/19/13, 3:30pm to 5:30pm
Location: GSBS Large Classroom S3.8371
Directions: Cynthia Mitchell Building, 6767 Bertner St., Houston, TX 77030
Facilitator: Dr. Todd Link
Speaker: Ronan O'Brien and Paul Belcher
Speaker Bio: see abstract in event description
Sponsor: Dr. John Ladbury
Contact: Deborah Watkins - (713) 834-6285 - firstname.lastname@example.org
CBSF Club Flyer September 19, 2013.pdf (PDF, 77 KB)
CBSF Club Abstract for Sept 19, 2013.pdf (PDF, 40 KB)
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