Research in my laboratory focuses on genetic regulation of pattern formation,
with emphasis on key regulators of limb, somite, and heart development.
Our primary experimental systems are the mouse and manipulation of embryonic
stem (ES) cells. At present we are in the process of developing new methodologies
to facilitate rapid manipulation of the mouse germline through modification
of ES cells. We plan to apply these methods to generate novel tools and
reagents in order to analyze mutations in genes that play critical roles
in development, differentiation, and disease.
Recent publications
- Kokubo H, Miyagawa-Tomita S, Saga Y, Johnson
RL (2005) Hesr1 and hesr2 are redundantly required
for cardiac chamber formation and arterial specification.
Dev Biol 278, 301–309.
- Kile BT, Hentges KE, Clark AT, Nakamura H,
Salinger AP, Box N, Liu B, Johnson
RL, Behringer
RR, Bradley A, Justice M (2003) Functional genetic
analysis of mouse chromosome 11: large-scale
isolation and rapid mapping of recessive mutations
using a balancer chromosome. Nature 425, 81–86.
- Kania
A, Johnson RL, Jessell TM (2000)
Coordinate roles for LIM homeobox genes in
directing the dorsoventral trajectory of motor
axons in the vertebrate limb. Cell 102,
161–173.
- M. P. Smidt, C. Asbreuk, J. J. Cox, H. Chen,
R. L. Johnson, J. P. H. Burbach
(2000) A second independent pathway for development
of mesencephalic dopaminergic neurons requires Lmx1b. Nature
Neuroscience
3, 337–341.
- C. L. Pressman, H. Chen, and R.
L. Johnson (2000) Lmx1b,
a LIM homeodomain class transcription factor,
is necessary for normal development of multiple
tissues in the anterior segment of the murine
eye. Genesis 26,
15–25.
- Lu MF, Pressman C, Dyer
R, Johnson RL,
Martin JF (1999) The function of the Rieger syndrome
gene, Pitx2, in left-right morphogenesis and
craniofacial development. Nature 401,
276–278.
- Aulehla A, Johnson RL (1999) Dynamic
expression of lunatic fringe suggests a link
between notch signaling and an autonomous cellular
oscillator driving somite segmentation. Dev
Biol 207, 49–61
- Evrard YA, Lun Y, Aulehla
A, Gan L, Johnson
RL (1998) lunatic
fringe is an essential
mediator of somite segmentation and patterning. Nature 394,
377–381.
- Kengaku M, Capdevila J,
Rodriguez-Esteban C, De La Peña J, Johnson
RL,
Izpisúa
Belmonte J-C, Tabin CJ (1998) WNT3a regulates
AER formation and utilizes an intracellular signaling
pathway distinct from the dorsoventral signal
WNT7a during chick limb morphogenesis. Science 280,
1274–1277.
- Dreyer S, Zhou G, Winterpacht
A, Zabel B, Cole W, Baldini A, Johnson RL,
Lee B (1998) Mutations in LMX1B cause abnormal
skeletal patterning and renal dysplasia in
nail patella syndrome. Nature
Genetics 19, 47–50.
- Chen H, Lun Y, Ovchinnikov
D, Kokubo H, Oberg KC, Pepicelli CV, Gan L,
Lee B, Johnson RL (1998)
Limb and kidney defects in lmx1b mutant mice
suggest an involvement of LMX1B in human nail
patella syndrome. Nature Genetics 19,
51–55.
- Johnson RL, Tabin
CJ (1997) Molecular models for vertebrate limb
development. Cell 90,
979–990.
- Levin M, Johnson RL,
Stern CD, Kuehn M, Tabin C (1995) A molecular
pathway determining left-right asymmetry in
chick embryogenesis. Cell 82,
803–814.
- Johnson RL, Laufer
E, Riddle RD, Tabin CJ (1994) Ectopic expression
of sonic hedgehog alters dorsal-ventral patterning
of somites. Cell 79,
1165–1173.
- Riddle RD, Johnson
RL, Laufer E, Tabin
C (1993) Sonic hedgehog mediates the polarizing
activity of the ZPA. Cell 75, 1401–1416.
Mailing Address:
Department of Biochemistry and Molecular Biology, Unit 1000
U.T. M .D. Anderson Cancer Center
1515 Holcombe Boulevard
Houston, TX 77030
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