Fee-for-Service structure
In general, the service/collaboration is provided on a first-come, first-serve basis. Priority will be given to projects in the following order: NIH funded, foundation funded, donor funded, and un-funded. Service may also be provided to outside collaborators at an increased charge. MDACC investigators will be given priority. An estimate of completion time for service, which mainly depands on the workload, will be given upon receipt of the request.
1. Microarray
The Cancer Genomic Lab currently provides service with the Whole genome 44K long oligo array from Agilent for both gene expression and array CGH. In the past, we have provided in-house arrays inclding a Pathway array with 1,500 gene. The genes that are involved in cell cycle, cell death, DNA repair, metastasis, signal transduction are included. The Pathway array is a long oligo (70mer) based array. The oligos were selected based on the regions that share limited homology with the family members. For gene identification of the spots and gene lists for our in-house arrays, please click on this site http://143.111.124.162/. For outside collaborators, please contact us for gene lists.
Our 4800 spot array
There will be different levels of service with different fees.
Level 1. Microarray experiments (Agilent whole genome array).
At this level of service, the investigators will provide the core lab with high quality total RNA or DNA. The Core Lab will evaluate the quality using Bioanalyzer from Agilent. The Core Lab Research Assistant will perform probe labeling, hybridization, and image acquisition. Contact Limei Hu at x53120 for questions
Level 2. Construction of customized arrays.
For investigators who are interested in building a specific pathway array, the Core Lab will work with the investigators in developing a schedule and charge plan. The charge will be dependent on the number of cDNA clones requested on the array and the number of arrays to be purchased. The Core Lab has built two customized arrays for MDACC investigators.
2. Quantitative real-time PCR assays for confirmation and for genetyping. contact David Cogdell at x53120 for questions.
3. RNA quality evaluation with Agilent Bioanalyzer
4. Agilent array CGH experiments
5. Protein lysate array. Contact David Cogdell for information.
6. Tissue array
--has been set up in Dr. Gregory Fuller's lab. Click Tissue array for link to Dr. Fuller's page Tissue array.