Back to M.D. Anderson Cancer Center

Back to W.M. Keck Center

Message from the Program Directors
Program Members
Institutional Vector Core
Adenovirus Section
Available Vectors
Service Request Forms
Retrovirus Section
Special Services
FAQ
Links
What's New
Becoming a Gene Therapy Patient
Recent Publications
Gene Therapy Links
Development Awards

 

 

 

Material Transfer Agreement - The uniform biological material transfer agreement.

Ad/CMV-LacZ

Ad/CMV-p53

Ad/CMV-GFP

Ad/RSV-Luc

Ad/CMV-p53-Flag

Ad/CMV-p53E4o

Ad/E1-

dl312

Ad/CMV-p21

Ad/E1-E4o

Ad/k-ras-sense

Ad/k-ras-antisense

Ad/GT-LacZ

Ad/GT-Luc

Ad/PO-Luc

Ad/RSV-hFix

Ad/PGK-GV16


Vector Name: Ad/CMV-LacZ

Expression Cassette: CMV-LacZ

Description: CMV-LacZ was placed in Adenovirus E1 region. This vector is usually used for evaluation the transduction efficiency of adenoviral mediated gene transfer. It is also frequently used as a control vector.

Constructor:

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference:

Return to Top


Vector Name: Ad/CMV-p53

Expression Cassette: CMV-human p53

Description: CMV-human p53 was placed in Adenovirus E1 region.

Constructor: Weiwei Zhang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference:

  1. Zhang WW et al., Cancer Gene Ther 1:5-13; 1994
  2. Zhang WW et al., Human Gene Ther 6:155-164;1995

Vector Map:

Return to Top


 Vector Name: Ad/CMV-GFP

Expression Cassette: CMV-Green Fluorescent Protein

Description: CMV-GFP is placed in Adenovirus E1 region. This vector is usually used for evaluation the transduction efficiency of adenoviral mediated gene transfer. It is also frequently used as a control vector.

Constructor: Ta-Jan Liu (mailto:tjliu@mail@mdanderson.org)

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference:

Return to Top


Vector Name: Ad/RSV-Luc

Expression Cassette: RSV-Luciferase

Description: RSV-Luc is placed in Adenovirus E1 region. This vector is usually used for evaluation the transduction efficiency of adenoviral mediated gene transfer. It is also frequently used as a control vector.

Constructor:

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone:(713) 792-8905.

Reference:

Return to Top


Vector Name: Ad/CMV-p53-Flag

Expression Cassette: CMV-human p53 attached with a Flag

Description: CMV-p53-Flag is placed in Adenovirus E1 region. The attached Flag will facilitate the detection of the transgene

Constructor:

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference:

Return to Top


Vector Name: Ad/CMV-p53-E4o

Expression Cassette: CMV-human p53

Description: CMV-p53 is placed in Adenovirus E1 region as Ad/CMV-p53. The vector has a modification in its E4 promoter region, i.e., the E4 promoter is replaced with GAL4/TATA. The vector can only grow in 293 cells stably transfected with GAL4/VP16

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Ji, L., et al., Gene Ther. 6:393-402;1999

Vector Map:

Return to Top


Vector Name: Ad/E1-

Expression Cassette: None

Description: The adenovirus E1 region is deleted (deleted region 456 ~ 3327 pb). The vector is generated by cotransfection of pXCJL with pJM17. It is used as vector control

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Ji, L., et al., Gene Ther. 6:393-402;1999

Vector Map:

Return to Top


Vector Name: dl312

Expression Cassette: None

Description: E1 deleted vector used as vector control. Deleted region bp 448~1349

Constructor:

Material Transfer Agreement Required: Permission from T. Shenk is required

Contact Person for Material Transfer:

Reference: Jones N & Shenk T, (1979) Cell 17:683-689

Return to Top


Vector Name: Ad/CMV-p21

Expression Cassette: CMV-human p21

Description: CMV-p21 is placed in Adenovirus E1 region.

Constructor:

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference:

Return to Top


Vector Name: Ad/E1-E4o

Expression Cassette: None

Description: E1 deleted vector. The deletion in E1 region is the same as Ad/E1-. The vector also contains a modification in the E4 promoter region. The E4 promoter is replaced with GAL4/TATA. The vector can only expanded in 293 cells transformed with GAL4/GV16

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Ji L et al., (1999) Gene Ther. 6:393-402;1999

Return to Top


Vector Name: Ad/K-ras-antisense

Expression Cassette: CMV-k-ras-antisense

Description: CMV-k-ras-antisense was placed in Adenovirus E1 region.

Constructor:

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference:

Return to Top


Vector Name: Ad/K-ras-sense

Expression Cassette: CMV-K-ras-sense

Description: CMV-K-ras-sense is placed in Adenovirus E1 region.

Constructor:

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference:

Return to Top


Vector Name: Ad/GT-LacZ

Expression Cassette: GAL4/TATA-LacZ

Description: GAL4/TATA-LacZ was placed in Adenovirus E1 region. This vector is used for evaluation the transduction efficiency of a binary adenoviral vector system. The LacZ will be expressed in presence of GAL4/VP16

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Fang B et al., (1998) J. Biol. Chem. 273:4972-4975

Return to Top


Vector Name: Ad/GT-Luc

Expression Cassette: GAL4/TATA-Luciferase

Description: GAL4/TATA-Luciferase is placed in Adenovirus E1 region. This vector is used for evaluation the transduction efficiency of a binary adenoviral vector system. The transgene luciferase will be expressed in the presence of GAL4/GV16

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Fang B et al., (1998) J. Biol. Chem. 273:4972-4875

Return to Top


Vector Name: Ad/PO-Luc

Expression Cassette: Luciferase cDNA without any promoter

Description: PO-Luc is placed in Adenovirus E1 region. This vector is used as control in a binary adenoviral vector system.

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Fang B. et al., (1998) J. Biol. Chem. 273:4972-4975

Return to Top


Vector Name: Ad/PKG-GV16

Expression Cassette: GAL4/VP16 fusion protein driven by mouse 3-phosphoglycerate kinase promoter

Description: PGK-GV16 is placed in Adenovirus E1 region. This vector is used for activating GAL4/TATA promoter in a binary adenoviral vector system.

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Fang B., et al., (1998) J. Biol. Chem. 273:4972-4975

Return to Top


Vector Name: Ad/RSV-hFix

Expression Cassette: RSV- human factor IX

Description: RSV-hFix is placed in Adenovirus E1 region. This vector can be used to monitor the transgene expression of a secretory protein.

Constructor: Bingliang Fang

Material Transfer Agreement Required: Yes

Contact Person for Material Transfer: Either Allan Prejusa or Trupti Mehta, phone: (713) 792-8905.

Reference: Fang B., et al., (1997) J. Virol. 71:4798-4903

Return to Top