Material
Transfer Agreement - The uniform biological material transfer
agreement.
Vector
Name:
Ad/CMV-LacZ
Expression Cassette: CMV-LacZ
Description: CMV-LacZ was
placed in Adenovirus E1 region. This vector is usually used for
evaluation the transduction efficiency of adenoviral mediated
gene transfer. It is also frequently used as a control vector.
Constructor:
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference:
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Vector Name: Ad/CMV-p53
Expression Cassette: CMV-human
p53
Description: CMV-human p53
was placed in Adenovirus E1 region.
Constructor: Weiwei Zhang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference:
- Zhang WW et al., Cancer Gene Ther
1:5-13; 1994
- Zhang WW et al., Human
Gene Ther 6:155-164;1995
Vector
Map:
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Vector
Name: Ad/CMV-GFP
Expression Cassette: CMV-Green
Fluorescent Protein
Description: CMV-GFP is placed
in Adenovirus E1 region. This vector is usually used for evaluation
the transduction efficiency of adenoviral mediated gene transfer.
It is also frequently used as a control vector.
Constructor:
Ta-Jan Liu (mailto:tjliu@mail@mdanderson.org)
Material
Transfer Agreement Required: Yes
Contact
Person for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference:
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Vector Name: Ad/RSV-Luc
Expression Cassette: RSV-Luciferase
Description: RSV-Luc is placed
in Adenovirus E1 region. This vector is usually used for evaluation
the transduction efficiency of adenoviral mediated gene transfer.
It is also frequently used as a control vector.
Constructor:
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone:(713) 792-8905.
Reference:
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Vector Name: Ad/CMV-p53-Flag
Expression Cassette: CMV-human
p53 attached with a Flag
Description: CMV-p53-Flag
is placed in Adenovirus E1 region. The attached Flag will facilitate
the detection of the transgene
Constructor:
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference:
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Vector Name: Ad/CMV-p53-E4o
Expression Cassette: CMV-human
p53
Description:
CMV-p53 is placed in Adenovirus E1 region as Ad/CMV-p53.
The vector has a modification in its E4 promoter region, i.e.,
the E4 promoter is replaced with GAL4/TATA. The vector can only
grow in 293 cells stably transfected with GAL4/VP16
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Ji, L., et al.,
Gene Ther. 6:393-402;1999
Vector
Map:
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Vector Name: Ad/E1-
Expression Cassette: None
Description: The adenovirus
E1 region is deleted (deleted region 456 ~ 3327 pb). The vector
is generated by cotransfection of pXCJL with pJM17. It is used
as vector control
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Ji, L., et al.,
Gene Ther. 6:393-402;1999
Vector
Map:
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Vector Name: dl312
Expression Cassette: None
Description: E1 deleted vector
used as vector control. Deleted region bp 448~1349
Constructor:
Material Transfer Agreement Required:
Permission from T. Shenk is required
Contact Person for Material Transfer:
Reference: Jones N & Shenk
T, (1979) Cell 17:683-689
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Vector Name: Ad/CMV-p21
Expression Cassette: CMV-human
p21
Description: CMV-p21 is placed
in Adenovirus E1 region.
Constructor:
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference:
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Vector Name: Ad/E1-E4o
Expression Cassette: None
Description:
E1 deleted vector. The deletion in E1 region is the same as
Ad/E1-. The vector also contains a modification
in the E4 promoter region. The E4 promoter is replaced with GAL4/TATA.
The vector can only expanded in 293 cells transformed with GAL4/GV16
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Ji L et al., (1999)
Gene Ther. 6:393-402;1999
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Vector Name: Ad/K-ras-antisense
Expression Cassette: CMV-k-ras-antisense
Description: CMV-k-ras-antisense
was placed in Adenovirus E1 region.
Constructor:
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference:
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Vector Name: Ad/K-ras-sense
Expression Cassette: CMV-K-ras-sense
Description: CMV-K-ras-sense
is placed in Adenovirus E1 region.
Constructor:
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference:
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Vector Name: Ad/GT-LacZ
Expression Cassette: GAL4/TATA-LacZ
Description: GAL4/TATA-LacZ
was placed in Adenovirus E1 region. This vector is used for evaluation
the transduction efficiency of a binary adenoviral vector system.
The LacZ will be expressed in presence of GAL4/VP16
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Fang B et al.,
(1998) J. Biol. Chem. 273:4972-4975
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Vector Name: Ad/GT-Luc
Expression Cassette: GAL4/TATA-Luciferase
Description: GAL4/TATA-Luciferase
is placed in Adenovirus E1 region. This vector is used for evaluation
the transduction efficiency of a binary adenoviral vector system.
The transgene luciferase will be expressed in the presence of
GAL4/GV16
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Fang B et al.,
(1998) J. Biol. Chem. 273:4972-4875
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Vector Name: Ad/PO-Luc
Expression Cassette: Luciferase
cDNA without any promoter
Description: PO-Luc is placed
in Adenovirus E1 region. This vector is used as control in a binary
adenoviral vector system.
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Fang B. et al.,
(1998) J. Biol. Chem. 273:4972-4975
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Vector Name: Ad/PKG-GV16
Expression Cassette: GAL4/VP16
fusion protein driven by mouse 3-phosphoglycerate kinase promoter
Description: PGK-GV16 is placed
in Adenovirus E1 region. This vector is used for activating GAL4/TATA
promoter in a binary adenoviral vector system.
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Fang B., et al.,
(1998) J. Biol. Chem. 273:4972-4975
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Vector Name: Ad/RSV-hFix
Expression Cassette: RSV-
human factor IX
Description: RSV-hFix is placed
in Adenovirus E1 region. This vector can be used to monitor the
transgene expression of a secretory protein.
Constructor: Bingliang Fang
Material Transfer Agreement Required:
Yes
Contact Person
for Material Transfer: Either Allan
Prejusa or Trupti
Mehta, phone: (713) 792-8905.
Reference: Fang B., et al.,
(1997) J. Virol. 71:4798-4903
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