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The Adenovirus-Based Vector Section produces vectors for pre-clinical studies.

Staff:


Bingliang Fang, M.D., Ph.D.

Dr. Fang has extensive experience in the field of gene therapy and gene transfer.

Trupti Mehta, Senior Research Assistant

has been working on adenovirus purification and quality control testing for more than five years. She is responsible for the following functions:

  • Growth and maintenance of the human embryonic kidney Cell line 293;
  • Expanding recombinant adenoviral vectors in 293 cells;
  • Purification of adenoviral vectors through cesium chloride gradient ultracentrifugation;
  • Performance and optimization of quality control tests required for gene vector preparations (detection of Replication Competent Adenovirus; titration of adenovirus particles and infectious units; mycoplasma testing; endotoxin testing);
  • Growth of mammalian cell cultures under BL2 and BL3 containment conditions;
  • Production and testing of Master Cell Banks (MCBs) and Working Cell Banks (WCBs);
  • Performing functional analysis of recombinant adenovirus in cultured cells;
  • Maintenance and up to date database of vector production, ordering and vector inventory;
  • Writing and maintaining protocols in accordance with GLPs; Interacting effectively with faculty, post-doctoral fellows, staff and graduate students;
  • Supervising the use of core facilities by core users; training core users in basic techniques for cell culture, virology, vector production and vector characterization;
  • Ordering supplies and minor items of equipment.

Alan Prejusa, Research Assistant II

has been working on adenovirus purification and quality control testing for more than two years. He is responsible for the following functions:

  • Growth and maintenance of the human embryonic kidney Cell line 293;
  • Expanding recombinant adenoviral vectors in 293 cells;
  • Purification of adenoviral vectors through cesium chloride gradient ultracentrifugation;
  • Performance and optimization of quality control tests required for gene vector preparations (detection of Replication Competent Adenovirus; titration of adenovirus particles and infectious units; mycoplasma testing; endotoxin testing);
  • Growth of mammalian cell cultures under BL2 and BL3 containment conditions;
  • Production and testing of Master Cell Banks (MCBs) and Working Cell Banks (WCBs);
  • Performing functional analysis of recombinant adenovirus in cultured cells;
  • Maintenance and up to date database of vector production, ordering and vector inventory;
  • Writing and maintaining protocols in accordance with GLPs;
  • Interacting effectively with faculty, post-doctoral fellows, staff and graduate students;
  • Supervising the use of core facilities by core users; training core users in basic techniques for cell culture, virology, vector production and vector characterization;
  • Ordering supplies and minor items of equipment.

Facilities:

The Adenovirus based vector facility consists of: · 682 square feet of general laboratory space, · two 110-sq. ft. hood rooms each containing a biosafety cabinet, · one 110-sq. ft. office, · 4 laminar flow hoods, and · 8 incubators for the construction and production of adenoviral vectors.

Services:

Production of adenovirus vector lots of cesium chloride purified vector. Vector lots are provided with a viral particle titer, infectious assay titration on permissive 293 host cells, RCA test report and Endotoxin test report. Aliquots may be purchased when an entire production lot (50 x 15 cm plates) is not required.

Adenovirus Vectors Currently Available

Service Request Forms (MDACC & Non-MDACC)

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