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The
Adenovirus-Based Vector Section produces vectors for pre-clinical
studies.
Staff:
Bingliang Fang,
M.D., Ph.D.
Dr. Fang has extensive experience in the field of gene therapy and
gene transfer.
Trupti
Mehta, Senior
Research Assistant
has
been working on adenovirus purification and quality control testing
for more than five years. She is responsible for the following functions:
- Growth and
maintenance of the human embryonic kidney Cell line 293;
- Expanding recombinant
adenoviral vectors in 293 cells;
- Purification
of adenoviral vectors through cesium chloride gradient ultracentrifugation;
- Performance
and optimization of quality control tests required for gene vector
preparations (detection of Replication Competent Adenovirus; titration
of adenovirus particles and infectious units; mycoplasma testing;
endotoxin testing);
- Growth of mammalian
cell cultures under BL2 and BL3 containment conditions;
- Production
and testing of Master Cell Banks (MCBs) and Working Cell Banks (WCBs);
- Performing
functional analysis of recombinant adenovirus in cultured cells;
- Maintenance
and up to date database of vector production, ordering and vector
inventory;
- Writing and
maintaining protocols in accordance with GLPs; Interacting effectively
with faculty, post-doctoral fellows, staff and graduate students;
- Supervising
the use of core facilities by core users; training core users in
basic techniques for cell culture, virology, vector production and
vector characterization;
- Ordering supplies
and minor items of equipment.
Alan
Prejusa,
Research Assistant II
has been working on adenovirus purification and quality control testing
for more than two years. He is responsible for the following functions:
- Growth and
maintenance of the human embryonic kidney Cell line 293;
- Expanding recombinant
adenoviral vectors in 293 cells;
- Purification
of adenoviral vectors through cesium chloride gradient ultracentrifugation;
- Performance
and optimization of quality control tests required for gene vector
preparations (detection of Replication Competent Adenovirus; titration
of adenovirus particles and infectious units; mycoplasma testing;
endotoxin testing);
- Growth of mammalian
cell cultures under BL2 and BL3 containment conditions;
- Production
and testing of Master Cell Banks (MCBs) and Working Cell Banks (WCBs);
- Performing
functional analysis of recombinant adenovirus in cultured cells;
- Maintenance
and up to date database of vector production, ordering and vector
inventory;
- Writing and
maintaining protocols in accordance with GLPs;
- Interacting
effectively with faculty, post-doctoral fellows, staff and graduate
students;
- Supervising
the use of core facilities by core users; training core users in
basic techniques for cell culture, virology, vector production and
vector characterization;
- Ordering supplies
and minor items of equipment.
Facilities:
The Adenovirus based vector facility consists of: · 682 square feet
of general laboratory space, · two 110-sq. ft. hood rooms each containing
a biosafety cabinet, · one 110-sq. ft. office, · 4 laminar flow hoods,
and · 8 incubators for the construction and production of adenoviral
vectors.
Services:
Production of adenovirus vector lots of cesium chloride purified vector.
Vector lots are provided with a viral particle titer, infectious assay
titration on permissive 293 host cells, RCA test report and Endotoxin
test report. Aliquots may be purchased when an entire production lot
(50 x 15 cm plates) is not required.
Adenovirus
Vectors Currently Available
Service
Request Forms (MDACC & Non-MDACC)
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