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Lymphoproliferative Disorder
Image Cytometry
Explanations & Definitions


HISTOGRAM CLASSIFICATION

The classification of cells by 6 types of histograms is the simplest and most generally accepted method in current use at M. D. Anderson Cancer Center.
 
Low grade
Intermediate grade
High grade
Histogram Patterns by Lymphoma Subtypes
 
Lymphoma Subtype*
L-I
L-IA
L-II
L-IIA
L-III
L-IIIA
Reactive
6
 
4
 
5
 
Small cleaved
33
1
1
   
1
Small lymphocytic
14
 
3
     
Mixed cell
5
 
5
5
   
Mantle cell 
1
 
3
     
Large cell
1
1
8
13
11
10
Ki-1        
4
Immunoblastic      
1
1
1
Lymphoblastic        
1
Peripheral T-cell      
1
   
Hodgkin's Disease
1
 
1
2
 
1
* According to the working formulation for non-Hodgkin's Lymphoma. 

Note: L-I, L-II, L-III subdivided by proliferation index: I < 1.2; II < 4.0; III > 4.0. L-IA, L-IIA, L-IIIA are as above but with hyperdiploid cells (DNA content > 5c).
 

Relationship between Histogram Patterns and Grade of Non-Hodgkin's Lymphoma and Reactive Lymph Nodes
 
Lymphoma Grade
L-I, L-IA
L-II, L-IIA
L-III, L-IIIA
Total
Low
54
14
3
71
Intermediate
2
19
18
39
High
0
0
8
8
Reactive
6
4
5
15
TOTAL       
133
Note: According to the Working Formulation for non-Hodgkin's lymphoma. 

Reference: Katz RL, Wojcik EM, Johnston DA: Image analysis in non-Hodgkin's lymphomas emphasizing applications in tissue procured by fine needle aspiration. In: GL Wied. ed. Compendium on the Computerized Cytology and Histology Laboratory, Science Printers and Publishers, Inc., St. Louis, MO, 1993.